2.2. Preparation of chitosan edible coating
Several chitosan with various DD was produced in the laboratory scale via deacetylation process of shrimp waste.
Two different DD of chitosans, i.e. 70% and 80% were selected for being used as edible coating of banana. The chitosan (CH) with various concentrations (1, 1.5, 2% w/w) was prepared by dissolving the corresponding amount of chitosan in the solution containing 0.6% w/w acetic acid. The mixture was heated to 90°C while stirring until more or less homogeneous. If TEA emulsifier was included in the solution, TEA of 0.15% w/w was added after fifteen minutes stirring at 90oC. The solution was then cooled down to a room temperature and stirring was continued until ten hours in order to achieve emulsion with good stability.