In order to determine the effect of the amount of C. tropicalis
cells lyophilized on the biosensor response the amount
of gelatin (4.42 mg cm
−2
) and glutaraldehyde (0.1%) on the
bioactive layer were kept constant therefore the amount of
C. tropicalis cells lyophilized was changed as 2.21, 4.42,
and 8.84 mg cm
−2
in the biosensor preparation. Fig. 1 shows