One gram of the leaf sample was
homogenized in 25 ml distilled water
with 3-4 drops of chloroform. The
homogenate was placed in 500 ml
conical flask. The filter paper strip
saturated with alkaline picrate solution
was placed in the hanging position
with the help of a cork stopper inside
the conical flask. The mixture was
incubated at room temperature (20
°
C)
for 20 – 24 hrs. The sodium picrate
present in the filter paper was reduced