Pellets were harvest and resuspended in sterile water and adjusted to 108 spores/ml.
To prepare A. solani spore suspensions, spores were inoculated in a petridish of PDA medium for 7 days at 28 C, 10 ml sterile water was added, and spores were scraped off and adjusted to 105 spores/ml.
Grading standards of decayed fruit were: 0, no disease spots; 1, area of disease spots < 1 cm2; 2, area of disease spots < 5 cm2; 3, area of disease spots < 10 cm2; 4, area of disease spots < 20 cm2; 5, area of disease spots > 20 cm2.
Rotting rate = (number of rotted fruits/total number of treated
fruits) 100.
Disease index =P(number of rotted fruits in one grade sequence number of grade)/(total number of treated fruits sequence number of the highest grade).
Preventive rate = (disease index of control fruits disease index of treated
fruits)/(disease index of control fruits) 100.
Pellets were harvest and resuspended in sterile water and adjusted to 108 spores/ml. To prepare A. solani spore suspensions, spores were inoculated in a petridish of PDA medium for 7 days at 28 C, 10 ml sterile water was added, and spores were scraped off and adjusted to 105 spores/ml. Grading standards of decayed fruit were: 0, no disease spots; 1, area of disease spots < 1 cm2; 2, area of disease spots < 5 cm2; 3, area of disease spots < 10 cm2; 4, area of disease spots < 20 cm2; 5, area of disease spots > 20 cm2. Rotting rate = (number of rotted fruits/total number of treatedfruits) 100. Disease index =P(number of rotted fruits in one grade sequence number of grade)/(total number of treated fruits sequence number of the highest grade). Preventive rate = (disease index of control fruits disease index of treatedfruits)/(disease index of control fruits) 100.
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