The PCR products of HBWB phytoplasmas were sequenced directly using six primers (350F, 520R, 788F, 920R, 1099F, and 1100R) that have been used to sequence the phytoplasma 16S rRNA gene (Namba et al. 1993). All 16S rRNA gene
sequences of HBWB isolated from several independent places were identical. The homology search had a 99.8%
similarity with the 16S rRNA gene sequence of onion yellows phytoplasma in Japan, and the HBWB phytoplasma
(accession no. AB242433) was identified as a strain of the “Candidatus Phytoplasma asteris” (Lee et al. 2004), based
on the 16S rRNA gene sequences analysis
The PCR products of HBWB phytoplasmas were sequenced directly using six primers (350F, 520R, 788F, 920R, 1099F, and 1100R) that have been used to sequence the phytoplasma 16S rRNA gene (Namba et al. 1993). All 16S rRNA genesequences of HBWB isolated from several independent places were identical. The homology search had a 99.8%similarity with the 16S rRNA gene sequence of onion yellows phytoplasma in Japan, and the HBWB phytoplasma(accession no. AB242433) was identified as a strain of the “Candidatus Phytoplasma asteris” (Lee et al. 2004), basedon the 16S rRNA gene sequences analysis
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