. The Ca concentrations of LB powder
and agar powder, which were determined by ICP spectrometry,
were 247 and 1410 ppm, respectively. These
data are consistent with the standard data in the Difco
Manual [10]. Therefore, Ca2þ concentrations in H2Oagar
and LB-agar, at maximum, were estimated to be
0.53 and 0.65 mM, respectively.