The mem-branes were incubated in blocking solution before incubation with
1:1000 dilution of primary antibodies (cyclin B1, cyclin D1, p53
and caspase-3; Millipore) overnight at 4C, then with secondary
goat anti-mouse IgG (H&L) alkaline phosphatase conjugated anti-body (Bio-Rad) for 90 min at room temperature.