37.813´E099° 51.588´. The study was conducted
in rainy season with an average rainfall of 136.6
mm in July and 212.7 in October. The averages of
temperature were 27.5 °
C in July and 27.8 °
C in
October. The averages of duration of sunshine were
4.6 hours/day in July and 4.0 hours/day in October.
The field facility was located at Agricultural Farm
of the Phetchaburi College of Agriculture and
Technology, Cha-am District, Phetchaburi Province,
Thailand.
In experiment 2, the effect of soaking seeds
of physic nut trees with colchicines on 0, 0.25,
1.25 and 2.50 mM was studied. Seeds of J. curcas
(Phechaburi variety) harvested from the physic trees
(in the plantation as described above) were used in
this experiment. This experiment was carried out
from August to October, 2010 in the green house.
The seeds from either treated inflorescences (from
Experiment 1) or treated seeds (from Experiment
2) were planted in the green house and many
characteristics were determined. These included
the ratio of female and male flowers after treating
by different colchicine concentrations in the
inflorescences in Experiment 1. The characteristics
of the germinated seedlings (both from treated
inflorescences and treated seeds) including plant
height, stomatal density and the sizes of guard cell were
determined in the laboratory at Animal Sciences
and Agricultural Technology, Silpakorn University,
Information Technology Campus, Cha-am
District, Phetchaburi Province, Thailand. Ploidy
level was determined by flow cytometry at Plant
Biotechnology Laboratory, Kasetsart University,
Kamphaeng Saen Campus, Kamphaeng Saen
District, Nakhon Pathom Province, Thailand.