influence on yield. The yield was linearly related
to incubation time, therefore providing a linear
increase in yield with incubation time for all
enzyme concentrations (Figure 2a). These results
agreed with Rastogi and Rashami (1999) that juice
yield was found to be a function of linear and
quadratic effects of enzyme concentration and
Shahaden and Abdullah (1995) that enzyme
concentration more affected banana juice yield
than other factors (pH and temperature).
Degradation of pectin by pectinase enzyme led to
a reduction of water holding capacity, and
therefore, free water released to the system caused
reduction of viscosity and increasing in yield
(Kashyap et al., 2001; Lee et al., 2006).
RSS was the calculated parameter from
yield and TSS, although R2 value of TSS was less
than 0.75, TSS was increased with enzymatic
treatment (Table 4). The R2 of RSS value in the
regression model (Table 3) was the highest of all
responses (0.924), indicating that the regression model of RSS could explain the reaction well. The
RSS value was significantly affected by first (p