Three samples were made by varying the autoclaving (hydrolysis) time of the solution. Three sample data are given in Table 03. Here we have got the highest percentage ethanol solution with the highest hydrolysis time. The same reason as like the Sulphuric acid concentration variation responsible here for the result. For a longer autoclaving time the hydrolysis process get more duration to occur. As a result the fermentable sugar concentration is increased in the solution which gives the higher percentage ethanol. Graphical presentation of those data is given in Figure 03.