Increasing pressure and time increased the oil yield, whereas increasing temperature did not have a significant effect. RSM-optimized conditions (45MPa, 70◦C and 224.5min) obtained by RSM for the range of variables predicted a yield of 27.0% oil, whereas the actual yield was 25.1±2.0%. Oil yield was further increased to 31.6% at the RSM-optimized conditions by increasing the SC-CO2 extraction time to 510min.