equipped with an HPX-87P Aminex column (Bio-Rad) and
a RID-10A refractive index detector (Shimadzu). The temperature was maintained at
80C, and water (Millipore) was used for the mobile phase at a flow rate of 0.6 mL/
min. All samples were filtered with a 0.45 mm disposal hydrophobic filter (Dismic-13
HP, Advantec, Toyo Roshi Kaisha, Ltd., Tokyo, Japan). The conversion of glucose or
mannose was calculated as the concentration of glucose or mannose released
compared to the initial glucose or mannose concentration in Japanese cedar
(Table S1). The efficiency of ethanol production was calculated as the ethanol concentration
in the total reaction volume compared to the theoretical ethanol yield
based on the amount of glucose and mannose present in the initial substrate, i.e., 1 g
of Japanese cedar (10% w/v). The theoretical yield is calculated from the stoichiometry
of the conversion of glucose and mannose to ethanol, which states that 1 mol
of glucose or mannose can yield 2 mol of ethanol. The data presented in the tables
are the mean values of three independent experiments.
equipped with an HPX-87P Aminex column (Bio-Rad) anda RID-10A refractive index detector (Shimadzu). The temperature was maintained at80C, and water (Millipore) was used for the mobile phase at a flow rate of 0.6 mL/min. All samples were filtered with a 0.45 mm disposal hydrophobic filter (Dismic-13HP, Advantec, Toyo Roshi Kaisha, Ltd., Tokyo, Japan). The conversion of glucose ormannose was calculated as the concentration of glucose or mannose releasedcompared to the initial glucose or mannose concentration in Japanese cedar(Table S1). The efficiency of ethanol production was calculated as the ethanol concentrationin the total reaction volume compared to the theoretical ethanol yieldbased on the amount of glucose and mannose present in the initial substrate, i.e., 1 gof Japanese cedar (10% w/v). The theoretical yield is calculated from the stoichiometryof the conversion of glucose and mannose to ethanol, which states that 1 molof glucose or mannose can yield 2 mol of ethanol. The data presented in the tablesare the mean values of three independent experiments.
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