The straw mushroom, Volvariella volvaceae is delicious and very popular among consumers. It is widely cultivated on the local and industrial scales in tropical and subtropical areas . In Thailand, spawn growers use animal manure, particularly horse manure as a mixture in spawn making. The objective of this research was to determine effects of five animal manures as an ingredient in the making of straw mushroom spawn. The experiment was conducted at Rajamangala University of Technology Suvarnnabhumi, Phra Nakhon Si Ayutthaya, Huntra by means of Complete Randomized Design (CRD) with five replications of five treatments. It was found that the mycelial growth on the horse and the goat manures for thirteen days was the most abundant. In addition, the growth of mycelia in the culture bowl which colonized four days after inoculation on the goat, buffalo and horse manures was rapid. As for the fructification level, six days after inoculation on the horse manure was found to be best with its value of 4.8. The results showed statistically significant differences.