For instance, immobilized Lipozyme RMIM lipase from Rhizomucor Miehei can be reused for only two cycles during transesterification of palm oil with methanol [45], Novozyme 435 in methanolysis of olive oil can be reused 5 cycles and the FAME yield reached 91.8% in 30 h. In contrast, TLL-epoxy can be recycled sixteen cycles with 82% FAME yield (95% of activity)
within 96 h and CALB-epoxy can be repeatedly used for up to fourteen cycles; keeping 93% of its initial activity.