The ability to create biofilms with typical characteristics that are consistently reproducible is of great importance to researchers in medicine, industry and academia. The technique described here was devised in an industrial laboratory to create uniform, reproducible biofilms for testing the effectiveness of cleansing and disinfecting products. Prior to the creation of this technique, industries wishing to test their products typically would spray planktonic cells onto sterile glass slides, dry them and then spray them with the selected product. Often the “kill” rate observed in these laboratory tests and the rate observed on naturally occurring microbial populations on surfaces were markedly different. This observation is explained by the unique properties of biofilms that include many phenotypic differences from planktonic cells including resistance to antimicrobic substances.