The study was performed in order to try to provoke the gonadotropin wave and ovulation in
common carp using a potent GnRH analogue, azagly-nafarelin (AZAGLY), without the use of
dopamine (DA) antagonists. Different doses, routes of delivery, and injection protocols were
applied, as well as different experimental conditions (laboratory, hatchery, and natural). It has been
shown that the best and the simplest way of AZAGLY administration was by intraperitoneal
injection. Despite dose (40 or 80 Ag/kg body weight) and number of injections (one or two),
maximal plasma carp-luteinizing hormone (cLH) concentrations never exceeded 40–50 ng/ml.
There was no priming effect on final plasma cLH concentrations. Using different treatment
protocols, it was possible to obtain 40–60% ovulated females in laboratory as well as hatchery
conditions. However, time of ovulation after AZAGLY treatment in some cases was less compressed
in comparison with Ovopel-treated (a local commercial product, containing a GnRH analogue plus
the dopamine antagonist, metoclopramide) fish. Egg quality was not altered by AZAGLY treatment
The study was performed in order to try to provoke the gonadotropin wave and ovulation in
common carp using a potent GnRH analogue, azagly-nafarelin (AZAGLY), without the use of
dopamine (DA) antagonists. Different doses, routes of delivery, and injection protocols were
applied, as well as different experimental conditions (laboratory, hatchery, and natural). It has been
shown that the best and the simplest way of AZAGLY administration was by intraperitoneal
injection. Despite dose (40 or 80 Ag/kg body weight) and number of injections (one or two),
maximal plasma carp-luteinizing hormone (cLH) concentrations never exceeded 40–50 ng/ml.
There was no priming effect on final plasma cLH concentrations. Using different treatment
protocols, it was possible to obtain 40–60% ovulated females in laboratory as well as hatchery
conditions. However, time of ovulation after AZAGLY treatment in some cases was less compressed
in comparison with Ovopel-treated (a local commercial product, containing a GnRH analogue plus
the dopamine antagonist, metoclopramide) fish. Egg quality was not altered by AZAGLY treatment
การแปล กรุณารอสักครู่..
