2.8. Gel-filtration chromatography
Size exclusion chromatography of the heteroglycan sulfate (OF4) on Sephadex G-200 column (90 × 2.6 cm) using 500 mM sodium acetate buffer (pH 4.0) as eluant was done as described (Mazumder et al., 2005). The column was calibrated with standard dextran sulfates (50 and 8 kDa; Sigma Chemical Company) and dextrans (10–500 kDa; a gift from Dr. Tapani Vuorinen).