Data analysis
Measures of genetic diversity based on allele frequencies, percentage polymorphic loci, mean number
of alleles, estimates of observed and expected heterozygosity, genetic distances and genetic identity
were calculated using software GENETIX 4.05 [19]. The structure among individual provenances was
estimated using Fst based on the method of Jackknife. This fixing index [20] is calculated based on the
formulae of [21]. Hierarchical analyses of molecular variance (Amova) [22] were calculated using the
program Arlequin, version 2.0 [23], in which significance levels for the populations values were
determined after 1000 permutations. The relationships between provenances were portrayed
graphically using Darwin 5.0 software [24] after the computation of genetic matrix [25] using UPGMA algorithm.