The produced enzymes were then used to release glucose and free amino nitrogen (FAN) from the food waste. Both glucose and FAN increased with increasing of food waste mass ratio from 5% to 15% (w/v). However, the glucose yield and starch conversion decreased from 0.434 g glucose/g food waste and 96.2% to 0.307 g glucose/g food waste and 68.1%, respectively, when the food waste mass ratio increased from 5% to 15% (w/v) probably because of the deactivation caused by high temperature or protease.