Fermentations were carried out in a 3 L BioFlo/celligen 115 fermenter (New Brunswick Scientific Co., INC, Edison, NJ) either spontaneously by the indigenous microbiota present on seeds (natural fermentation, NF) or with 2% (v/v) of L. plantarum suspension (108 CFU/mL) (LPF), at 37 C and continuous agitation at 350 rpm for 96 h.
fermenter