Nisin and reutericyclin were mixed with endospore suspensions
(OD600 2.0) at 16X the endospore MIC values (16 mg L1
and
6.4 mg L1
, respectively). Endospore suspensions with antimicrobials were heat-sealed in borosilicate glass capillary tubes (Disposable Micropipets, Fisher Scientific, Toronto, Canada) and kept on ice
overnight until treatment. Thermal treatments were performed in
an oil bath. Samples were placed into pre-heated canola oil at 90
C,
100
C, and 120
C for up to 60 min. Initiation of thermal treatment of
samples was taken as the moment samples were placed into preheated oil, and designated as 0 min treatment time. Samples were
stored on ice for