sterilized at 121 °C with pressure of 1.2 kg/cm2
for 15 min. The materials
were grown under the same conditions as those for the proliferation
culture. Each treatment contained 30 shoots. After 12 months of culture,
all explants from each treatment were evaluated for growth and recovery
in the same manner as previously described.
RESULTS AND DISCUSSION
Effects of ABA and Paclobutrazol on In Vitro Shoot Conservation of
Mangosteen and Longkong
The shoot growths of both species were suppressed by the
application of ABA and paclobutrazol into the culture medium (Tables 1
and 2). In case of mangosteen, after 12 months of conservation, it was
found that the controls had the highest viability at 99.33 %, while shoots
cultured in the medium with ABA seemed to survive at higher rates than
those cultured in the paclobutrazol medium (Table 1). Growth of shoots
cultured in medium containing ABA and paclobutrazol was markedly
suppressed when compared with those in controls (Table 1 and Figure 1).
However, the new shoots produced during conservation in the treatment
tested were not significantly different. The average shoot number ranged
from 2.0 to 3.6 shoots (Table 1). After 2 months of culture, most of the
shoots cultured in the ABA treatment swelled at the cut end of the shoot.
The browning of the mangosteen shoot was observed in the shoots
cultured with 2.0 mg/l paclobutrazol (Figure 1).