The bacterial flora was enumerated by direct plating technique
on plat count agar (PCA) after tenfold dilution wherever
needed. Inoculated plates were incubated at 35oC and total colony forming units (CFU) of the bacterial flora were enumerated after 24, 48 and 72 h, and 7 days—Method 9215B.
Total and faecal coliforms were determined by the membrane
filter procedure Methods 9222B and 9222D, respectively.
- Chemical oxygen demand (COD) was determined using the
Titrimetric Method 5220C.