Tomato (Solanum lycopersicum) varieties used included the T56 industrial
processing genotype as the background for the P119, TFM7, and
2A11 DET1 downregulated genotypes (Davuluri et al., 2005). The hp2j
Analysis of DET1 Downregulated Tomato Fruit 1209
mutant allele in the Moneymaker variety background and the hp2 mutant
in the San Marzano background were also used. Details of the phenotypes
and recommendations for growth can be found on the Tomato
Genetic Resource Centre (TGRC) at www.tgrc.ucdavis.edu. Seeds for
these two hp mutant varieties and their respective backgroundswere also
obtained from TGRC. Tomato plants were glasshouse grown from seed
under supplementary lighting. Routinely, six plants per genotype were
grown in a randomized manner concurrently with their respective backgrounds.
Figure 1 illustrates the designations used to defined the stages
of fruit development and ripening used in this study. The breaker stage
representing the initial onset of ripening was taken as the first sign of color
and ripe fruit as 7 d postbreaker.
Molecular Analysis
Measurement of Gene Expression by