Safflower florets powder (1g)
Suspended in 20 mL sodium carbonate
Stirred (30 min)
Centrifuged (3500 rpm for 30 min)
Supernatant–I
Re-extracted (Residues + 20 mL sodium carbonate)
Centrifuged at 3500 rpm for 15 min)
Supernatant – II
Remove residues
Mix supernatant I and II
Acidified (citric acid 0.5 %)
Adsorption of carthamin (cellulose powder - 0.5gm)
Stirred (30 min)
Centrifuged (3500 rpm for 15 min)
Packed
Freeze dried
Supernatant discard
Figure 2:Flow sheet for the extraction of carthamin from safflower petals.