Recently, a chemiluminescent immunoassay has been shown to be
more sensitive than the conventional colorimetric method(s), and does
not require long incubations or the addition of stopping reagents, as is
the case in some colorimetric assays. Among various enzyme assays
that employ light-emitting reactions, one of the most successful assays
is the enhanced chemiluminescent immunoassay involving a
horseradish peroxidase (HRP) labeled antibody or antigen and a
mixture of chemiluminescent substrate, hydrogen peroxide, and
enhancers.