using
V. parahaemolyticus as host. Five hundred milliliters of water were filtered
sequentially by 3 μm and then by 0.45 μm pore-size polycarbonate
membranes (Millipore, Billerica, USA). Filtered water was added to
500 mL of TSB with double concentration and to 1 mL of bacterial culture.
The mixture was incubated at 25 °C for 18 h at 80 rpm, and then