In this study, different pesticides were used to evaluate their
effects on alcoholic fermentation and growth of the yeast S. cerevisiae.
Omethoate, triadimefon, chlorothalonil and cyhalothrin
were chosen as the model pesticides mainly because their wide use
on grape planting and their residues were easily detected in grapes
and grape juices. Roughly speaking, omethoate is an organophosphorus
systemic insecticide; triadimefon is a triazole systemic
fungicide; chlorothalonil is an organochlorine non-systemic
fungicide, and cyhalothrin is a pyrethroid and non-systemic
insecticide. Then the pesticide with inhibition effect was pretreated
by its corresponding degrading-enzyme secreted from recombinant
Bacillus subtilis WB800 to investigate the elimination of
its adverse effect on winemaking.