To obtain blood samples, fish were caught gently
in a small scoop net and then quickly taken out
from the water and fish were anaesthetized by
5mg/l of clove oil to avoid any handling stress in
order to minimize an error in normal blood values.
Blood was obtained from the renal artery by
cutting off the tail peduncle. Blood glucose was
measured by using a digital blood glucose kit. A
drop of blood sample was placed on the strips
connected to the GLUCOLABTM Auto-coding
blood glucose test meter and results were recorded.
The values were expressed in mmol/l. Hematological
analyses were carried out by standard
methods suggested by Blaxhall and Daisley
(1973). To examine red blood cell (RBC) and
white blood cell (WBC) counts, collected blood
was gently pushed into sterilized eppendorf tubes
containing anticoagulant (EDTA) to give a final
concentration of 5mg EDTA per cm3 blood.
Blood samples were mixed gently and counts
were made using a Neubauer hemocytometer.