One milliliter of different
concentrations of extracts (1, 10, 100 and 200 lg/mL) prepared
in methanol was added to 0.5 mL of a 0.2 mmol/L DPPH
methanolic solution. The mixture was vigorously shaken and
left standing at room temperature for 30 min. The absorbance
of the resulting solution was then measured at 517 nm after
30 min. The antiradical activity (three replicates per treatment)
was expressed as IC50 (lg/mL), the concentration required to
cause a 50% DPPH inhibition.