Numerous Bial’s reagents were prepared with varying HCl
concentrations (0 Me12 M) and FeCl3 (0.01e0.6 g). The reagent
was added to a fixed xylose solution (0.02% w/v) and heated in
boiling water bath (100 C) at varying reaction times (1e60min).
The resulting green-colored solution containing the complex
was scanned on a double beam UVeVis spectrophotometer to
obtain its absorption spectra.