Determination of antagonistic activities of Allium
cepa
Disc diffusion method was used. The discs
were prepared with Whattman paper which is around
6mm in diameter. The paper was sterilized using hot air
oven at 1600C for 2 hours. The prepared paper was
aseptically soaked into the extract. Sterile nutrient agar
was aseptically poured into sterile Petri dishes and
allowed to solidify. Test organisms were taken from
overnight culture to inoculate the dried agar plates by
streaking. Sterile forceps was used and discs were
aseptically place on the surface of Agar plates.
Ciprofloxacin was used in the nutrient agar plates as a
control and the plates were incubated at 370C for 24
hours. The plates were examined for zones of
inhibitions.