For amylase and
xylanase assay, the substrate used was 1% (w v
1
) soluble starch
(Fisher Scientific) and 1% beechwood xylan (Sigma), respectively;
while 1.25% (w v
1
) of carboxymethylcellulose (CMC) (R&M Chemicals) was used to determine cellulase activities. Control was
prepared by substituting enzyme extract with plain actinomyces
broth (Difco) at same volume.