2.1. Broodstock rearing
The experiment was located in the field research facility of the
Department of Aquaculture, Faculty of Fisheries and Marine Science,
Bogor Agricultural University, Indonesia. Male and female tilapia
broodstock were obtained from the Wanayasa Nila Nirwana Development
Center, the Department of Fisheries ofWest Java Province (BPBIAT
Wanayasa,West Java, Indonesia). The fishwere acclimatized to the local
rearing conditions for two weeks before the start of the experiment.
During this period, female and male fish were kept separately in three
outdoor tanks (3m× 2m× 0.7 m) previously filled with 3000 L chlorinated
and aerated water at a density of 40 fish per tank for female
broodstock and 20 fish per tank for male broodstock