A modified method for measuring RS in dietary fiber residues
from various sources developed by Saura-Calixto and others
(1993) involves mixing fiber residues with KOH, acetate buffer,
and HCl. After incubation with amyloglucosidase samples are
centrifuged and diluted with distilled water. RS is calculated as
glucose (mg) × 0.9. Advantages of the method are the use of small
amount of sample, less reagents and elimination of drying.