) Place one spotted TLC plate in the TLC chamber, cover with a watch glass and allow the
Developing solvent to move through the plate until it is approximately 0.5‐1 cm from the top. Do not
disturb the chamber while the plate is being developed!!!
5) Remove the plate from the chamber and mark the solvent front with a pencil. Allow the
plate to dry for a few minutes. Draw the picture of TLC plate as you observed in your note book