The U5 enzyme maintained almost the same fructofuranosidase
activity at both sucrose concentrations, but showed higher
transfructosylating activity at 50% sucrose. On the other hand,
the V10 enzyme showed low FTA activity on 2% sucrose, but at
50% sucrose concentrations the FTA activity was almost eight
times the FA activity, so although this enzyme shows some
fructofuranosidase characteristics, it may have a very particular
kinetic behaviour.
Other valuable information was that no enzyme showed
remarkable inulin hydrolysing activity, so they could not be
characterized as inulinase enzymes. At 50% sucrose concentration,
the V2 enzyme showed high transfructosylating activity,