The resulting LvRab7 (618 bp), TSV (920 bp) and actin
(550 bp) PCR products were analyzed by gel electrophoresis
using a 1.5% agarose gel. The gel was stained with
ethidium bromide and photographed using a short enough
exposure time that the band intensities were not saturated.
Densitometric scanning of the photograph was performed,
and the band intensities were quantified using the Scion
image analysis program. The signal was corrected for the
background. The band intensity of TSV or LvRab7 of each
sample was normalized against the band intensity of the
actin of that sample to give the arbitrary units of the relative
expression of TSV genome replication and LvRab7.