China aster, Callistephus chinensis, is widely grown in Myanmar as an ornamental plant. Symptoms of flower virescence were
observed in diseased China aster plants in Yezin, Myanmar. The presence of a phytoplasma was detected and identified by applying
Polymerase Chain Reaction (PCR)/ Restriction Fragment Length Polymorphism (RFLP) techniques and sequencing the 16S ribosomal
DNA. The phytoplasma was identified as belonging to ribosomal subgroup 16SrII-A, never reported before in China aster. It showed
almost 100 % similarity with a 16S rDNA sequence of sunn hemp witches’ broom phytoplasma (AB558143), which belongs to the peanut
witches’ broom phytoplasma group. This is the first record of China aster as a new host for a subgroup 16SrII-A phytoplasma.
Key words: China aster, Phytoplasma, 16S rDNA, RFLP.
China aster, Callistephus chinensis, is widely grown in Myanmar as an ornamental plant. Symptoms of flower virescence wereobserved in diseased China aster plants in Yezin, Myanmar. The presence of a phytoplasma was detected and identified by applyingPolymerase Chain Reaction (PCR)/ Restriction Fragment Length Polymorphism (RFLP) techniques and sequencing the 16S ribosomalDNA. The phytoplasma was identified as belonging to ribosomal subgroup 16SrII-A, never reported before in China aster. It showedalmost 100 % similarity with a 16S rDNA sequence of sunn hemp witches’ broom phytoplasma (AB558143), which belongs to the peanutwitches’ broom phytoplasma group. This is the first record of China aster as a new host for a subgroup 16SrII-A phytoplasma.Key words: China aster, Phytoplasma, 16S rDNA, RFLP.
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