Fluorescence Microscopy
Cells were treated with rhodamine-labeled 70 nm SiO2 particles (Z-PS-SIL-RFP-
0,07; Postnova Analytics Landsberg, Germany) and fixed in ice cold 100% methanol.
The nuclei were stained with DAPI (Molecular Probes). The stained cells
were visualized using an Olympus 1 × 50 fluorescent microscope and a Hamamatsu camera. Images were analyzed using ImageJ software.