Polyphenol extracts from the apple DF were prepared for the total
phenolic content and individual phenolic analysis following the
procedure of Sun-Waterhouse, Farr, et al. (2008). Freeze-dried apple
fibre (100 mg) was mixed with 0.1 M NaOH (at a concentration
of 10 mg/ml, under N2), vortexed, acidified to pH 3 with 1 M HCl,
and then extracted with ethyl acetate (1:1) three times. The upper
ethyl acetate fraction was collected after centrifugation (3000g,
5 min). The three ethyl acetate extracts were combined and dried
(under N2).
Polyphenol extracts from the apple DF were prepared for the total
phenolic content and individual phenolic analysis following the
procedure of Sun-Waterhouse, Farr, et al. (2008). Freeze-dried apple
fibre (100 mg) was mixed with 0.1 M NaOH (at a concentration
of 10 mg/ml, under N2), vortexed, acidified to pH 3 with 1 M HCl,
and then extracted with ethyl acetate (1:1) three times. The upper
ethyl acetate fraction was collected after centrifugation (3000g,
5 min). The three ethyl acetate extracts were combined and dried
(under N2).
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