β-Glucan-rich fractions (BGRFs) from L. edodes mushrooms were incorporated into the formulation of gluten-free rice noodles in order to improve their quality attributes as well as to provide β-glucan derived health benefits. While rice suspension with BGRFs exhibited reduced values of water hydration and pasting parameters, the use of BGRFs increased the thermo-mechanical properties of rice flour in a dough system, consequently producing rice noodles with increased extensibility and firmness. These textural characteristics were favorably correlated to the reduced swelling index and cooking loss of BGRF-incorporated rice noodles.