2.8. Mycoflora of hot water treated fruit
Mycoflora of matured green naturally infected fruit exposed to
HWT (53 C, 20 min) was assessed after 14 days. Using a sterile
scalpel, ten tissue squares (1 cm2) were randomly taken from the
fruit skin. Tissue pieces were surface sterilized with chlorox solution
for 30 s and rinsed three times in SDW. Five tissue pieces were
planted on each of three PDA plates and incubated for 3e7 days at
25 C. Fungal colonies developing from the tissues were isolated
and identified.
2.9. Statistical analysis
All data were subjected to analysis of variance (ANOVA) and the
significant differences among treatments were separated by
Tukey's test at P ¼ 0.05 using SPSS Statistics version 16.0 for Windows
(IBM, Armonk, NY, USA).