The 50 g of dried leaves powder was thoroughly mixed with
200 mL methanol in triplicate conical flasks. The flasks containing
extract were heated on boiling water bath for 1 h,
wrapped with aluminum foil and kept at room temperature for
5 days with occasional shaking. After stipulated time, the liquid
extract of flasks was transferred to falcon tubes and subjected
to centrifugation at 5000 rpm for 10 min. The clear supernatants
from the falcon tubes were shifted to pre-weighed beakers, and
allowed to evaporate the solvent in hot water bath to get dried
methanol free extract of E. tereticornis.