The finding of this study reveals that the supercritical extraction yield of A. Malaccensis increases with
increasing pressure and temperature. Supercritical extracte of A. Malaccensis is an effective cytotoxic
agent. Its toxicity increased with temperature increment up to 50ºC and lowering the pressure to 20.7
MPa. The first 10 min fraction of supercritical extraction possesses potent toxicity towards HCT116
cancer cells with an IC 50 of 4 ug/ml. This result demonstrates that temperature is an effective factor for
the extraction yield and for the extracting of cytotoxic compound.