Figure 1 Effect of oxygenation conditions and inoculation densities
on growth, citric acid production, and lipid production by Y. lipolytica
grown in molasses. Strain JMY4086 was grown in a molasses medium
and fed with crude glycerol. Growth is expressed as a cell dry weight,
b citric acid production, and c lipid production. X biomass, CA citric
acid, L lipids, 1 low-density inoculum/unregulated oxygenation
condition (filled circles), 2 low-density inoculum/regulated oxygenation
condition (filled squares), 3 high-density inoculum/unregulated
oxygenation condition (filled triangles), 4 high-density inoculum/
regulated oxygenation condition (filled diagonals). The low-density
and high-density inocula had optical densities of OD600 = 1 and
OD600 = 6, respectively. For the unregulated oxygenation condition,
stirring speed was a constant 800 rpm and the aeration rate was
1.5 L/min. For the regulated oxygenation condition, dissolved oxygen
was maintained at 50% saturation and the aeration rate was 0–3.5 L/
min. All the results presented are the mean values ± SD for two
independent biological replicates.