for 30 min. They were trimmed to a
three node sized segments and
worked out further under clean ben-
ch for aseptic operation after they
were treated with 0.05% mercuric
chloride (HgCl ) for 1, 2 and 3 min, 2
respectively, for surface disinfection.
After surface disinfection, they were
rinsed for 2-3 min in sterile water
then cultured on ¾ strength MS
media containing 2mg/l BAP (Kashif
et al., 2005).