However, in our experiment the most saturated fat (RPF) resulted in a 2.1 L CH4 /kg DMI decrease compared with CON whereas the more unsaturated diet WCR only had a reduction of 0.8 L CH4 /kg DMI compared to CON. Unsaturated fat is expected to be more potent than saturated fat in reducing CH4 production however, in the present study the more saturated RPF and RPFA revealed the lowest CH4 production related to DMI and GE intake. As CH4 production per kg DM decrease with increased DMI, the numerically higher DMI of RPF and RPFA compared to WCR could possibly explain the lower CH4 production.