comparable to other studies. Li et al. (2009) reported the sensitivity of
the LAMP detection was determined at 35 CFU/250 mL of enriched
culture for artificial contamination of minced meat or raw milk by
Salmonella strains, Wang et al. (2008b) reported the detection limits of
LAMP assay for Salmonella were 100 fg DNA/tube, Vantarakis et al.
(2000) reported 10–100 cells/mL of Salmonella and Shigella in
homogenate mussels were detected by the mPCR. The sensitivity of
mLAMP assay in our study was found to be 100 fg DNA/tube, which is
identical to those described by Wang et al. (2008a,b), and an initial
inoculation level of Salmonella and Shigella as low as 5 CFU/10 mL in
milk could be identified. Compared with traditional method, the whole
process of mLAMP assay is not more than 20 h including enrichment of
pathogenic bacteria, which greatly saved detection time