Rice starch was used to investigate the effect of maltogenic amylase
on the changes in the proportion of amylose and amylopectin. Rice
starch (5 mg) was pre-incubated with 50 μL of DMSO and 850 μL of
sodium acetate buffer (50 mM, pH 5.5) at 95 °C for 3 min. Then,
100 μL of TfMA at 0.175 U/mLwas added. The reactions were performed
at the same temperature for 30, 60, 90 and 120 min.